1 04 Researches Regarding Cholera : The Blood. [part i. 



right femoral vein. The evacuation employed was that which had afforded the materials 

 for injection in Experiments XIII and XXII, and had been kept for 25 days at 

 the time the solution was prepared. The proportions of the water and choleraic fluid 

 in the solution were 20 minims of the latter to 1 ounce of the former. 



The injection was successfully performed, and the dog rapidly recovered from the 

 influence of the chloroform ; but the respiration continued to be hurried and somewhat 

 irregular for nearly an hour. This symptom, however, passed off, and the dog 

 appeared not very much affected. He refused all food, however, and began to suffer 

 from diarrhoea, passing evacuations, the first of which were normal in aspect, while 

 the subsequent ones became more and more mucus and blood-streaked. Four hours 

 and a half after the operation, he was observed to suffer from rigors, and these 

 continued to occur for the next three hours, at the close of which period he died. 



A 'post-morteTYi examination was performed two and a half hours after death. 

 Rigor 'mortis had not set in. The body was still slightly warm and the abdomen 

 was not distended. The parts around the wound in the thigh appeared quite healthy, 

 and the vein above the ligature was normal in aspect and distended with fluid blood. 

 On opening the abdomen the cavity was found to be free of fluid, and the peritoneum 

 seemed to be quite healthy. The stomach contained glairy fluid mingled with bile. 

 The interior of the small intestines was extremely congested, and the mucous 

 membrane was of a deep pink colour from the duodenum to the ileo-coecal valve. 

 They were full of a red fluid mixed with grumous matter. This material when 

 subjected to immediate microscopic examination showed no distinct traces of blood 

 cells, but contained more amorphous particles with some oil globules and a few 

 epithelial cells. The large intestine was pale and almost empty. The liver was 

 abnormally friable, and showed numerous yellow fatty spots scattered over the 

 surface and extending into the substance. The gall-bladder was full but not dis- 

 tended. The spleen appeared healthy. The medullary portion of the kidneys was 

 very red, while the cortical substance was of a pale yellow tint and fatty aspect. 



On opening the thorax, no signs of pleurisy could be detected. The lungs 

 were totally collapsed, airless and almost bloodless. The pericardium was healthy, 

 and contained no fluid. The right cavities of the heart were full of dark coagula 

 and fluid blood. The left cavities were empty, and the ventricle was strongly con- 

 tracted. 



(6) — The solutionis having been prepared twenty-four hours previously. 



Experiment XXXVIII. — The dog which had on the preceding day been made 

 the subject of Experiment XXXIV without appearing in any way affected by it 

 was again put under the influence of chloroform, and two drachms of the solution 

 previously employed, but which was now in an active state of decomposition, con- 

 taining innumerable monads, bacteria and vibriones, were injected into the left 



