PART I.] Injections of Solutions Prepared Forty-eight Hours before Use. 105 



femoral vein. The animal recovered quickly, as on the previous occasion, remained 

 well afterwards, and was subsequently the subject of Experiment XIV. 



Experiment XXXIX. — A young healthy dog was put under chloroform, and 

 half an ounce of an aqueous solution of choleraic material injected into the right 

 basilic vein. The solution had been prepared twenty-four hours previously, and was 

 derived from the same evacuation as employed in Experiment XXX. The operation 

 was successfully performed, and the dog rapidly recovered from the influence of the 

 chloroform. Shortly afterwards well marked rigors occurred, and the animal died 

 four hours subsequently, having passed one liquid evacuation during the interval. 



A post-mortei7i examination was performed one hour and three-quarters after 

 death. The body was still warm, and 7ngor mortis just commencing. The peritoneal 

 cavity contained no fluid and the membrane was healthy. The mucous coat of the 

 intestines both large and small was injected almost universally, but the contents of 

 the guts were of a yellowish-white tint, only here and there showing a pinkish 

 tinge. In these coloured portions the consistence of the mucous matter of which 

 they were composed was more fluid than elsewhere. Several tortuous patches of small 

 vessels were visible on the surface of the liver ; they were gorged with blood, but 

 the hepatic cells in their neighbourhood appeared to be unaffected in any way when 

 examined microscopically. The spleen and kidneys were healthy. 



On opening the thorax the pleural cavities were found to be quite healthy. The 

 lungs were collapsed, airless and bloodless, and several dark extra vasated patches were 

 present in each. The heart was healthy. The right cavities were full, and the left 

 almost empty. Specimens of blood were obtained from each side of the heart, but 

 no distinct traces of monads or bacteria could be detected in them at the time, nor 

 were any observed to have developed in them two days subsequently, when filaments 

 of fungi had crept into the preparations through cracks in the covers of the wax-cells 

 in which they were contained. 



(c) — The solutimis having been prepared forty-eight hours previously. 



Experiment XL. — The dog employed in Experiment XXXVI, but which now 

 appeared to be in perfect health, and with the wound in the right foreleg clean and 

 healing, was again put under the influence of chloroform, and five drachms of the 

 solution used in the preceding experiment, but which had now been kept for 

 48 hours, were injected into the left median vein. The fluid was thoroughly 

 shaken up previous to injection, in spite of which the animal rapidly recovered from 

 the influence of the chloroform, and began to run about as though nothing had 

 happened. He continued in apparent good health during the next two days, and was 

 then made the subject of Experiment VI. 



