T I o Researches Regarding Cholera : The Blood. [part i. 



intestine generally was much disorganized. The other abdominal and thoracic organs 

 were healthy. 



Experiment LII. — Immediately on completion of the preceding experiment, another 

 huge pariah dog was put under chloroform, and six drachms of the same decomposing 

 fluid, swarming with bacteria, injected into its right femoral vein. The animal ceased 

 to respire almost immediately, and efforts to restore it were in vain, although it had 

 nearly come from under the influence of the anaesthetic, and none had been admini- 

 stered for some time. 



The viscera were forthwith exposed ; the right side of the heart was enormously 

 distended, and the left contained a little blood. The mesenteric glands were pink, and 

 contained red blood-corpuscles. The liver was of a very dark colour and gorged with 

 blood ; other viscera healthy. 



Three wax-cell preparations of the blood were made ; one from the right side of 

 the heart, another from the left side, and a third from the left femoral. Of these, in 

 the first only could active bacteria be distinguished when examined immediately after 

 the specimens were prepared ; nor did any appear in the other two for some hours ; 

 next morning, however, all three contained an abundance of moving bacteria. 



Two wax-cell preparations were also made of the fluid squeezed out of an axillary, 

 and out of a mesenteric gland, both of which contained numerous active bacteria, 

 and monads from the first, and their numbers increased greatly during the following 

 twenty-four hours. In about four days the activity of the monads an.d bacteria ceased, 

 motionless molecules alone remaining in the blood, as well as in the gland-juice 

 preparations. 



(cZ) — Tlie organic material injected being three days old. 



Experiment LIII. — In order to complete the series of filtered and unfiltered, 

 fresh, and decomposed solutions of a simple organic liquid, two drachms of the 

 decomposed watery solution of fowl's blood, which had been used in previous experi- 

 ments, and had, by this time, acquired an intensely putrid odour, and swarmed with 

 active bacteria, were injected, without previous filtration, into the right femoral vein. 



Owing to various accidents the dog had to be kept under the influence of 

 chloroform for a considerable time. Its respiration twice entirely ceased, and was, 

 on each occasion, restored by mechanical means. At the close of the operation the 

 .abdomen was extremely distended. 



In spite of these adverse circumstances, the animal on the second day was quite 

 lively, and partook of its food freely, although the wound did not present a healthy 

 appearance ; but on the fifth day it was so far recovered as to be considered fit to 

 undergo another operation, from which he also recovered and eventually escaped 

 {vide Exp. XLIII). 



