112 , Researches Regarding Cholera : The Blood. [part l. 



A wax-cell preparation of the blood was kept under observation for three days, but 

 no bacteria nor any other organisms developed. A similar preparation was made by 

 squeezing some fluid out of a mesenteric gland ; here also no monads or bacteria could 

 be detected during the period above named. 



Experiment LVII. — The subject of Exp. XX, a large pariah dog, into whose 

 right femoral vein decomposing choleraic fluid had been introduced five days previously 

 without producing serious illness, was put again under the influence of chloroform, 

 and six drachms of a solution of normal foecal matter injected into the other femoral 

 vein. Neither was the animal much affected by this, and four days afterwards appeared 

 to be in perfect health, when it was killed under chloroform and immediately 

 examined. 



The thoracic and abdominal viscera were normal, and the mucous coat of the 

 intestines quite unaffected. A wax-cell preparation of blood from the heart appeared 

 to be a perfectly healthy sample ; there were no bacteria visible, nor were any developed 

 during the following two days. A similar preparation was made of the fluid in the 

 mesenteric glands (which was very abundant) ; on the first day no distinct bacteria were 

 visible, but on the following morning the preparation was crowded with very active 

 large bacteria, together with long, active, and still oscillatoria-like vibriones, such as 

 are depicted in Fig. 1, page 88; on the third day these organisms were all motionless 

 and degenerated into a beaded leptothrix network (Fig. 2, page 88). 



{e)—-The injected material being four days old. 



Experiment LVIII. — A large healthy pariah dog was brought under the influence 

 of chloroform, and four drachms of a solution of healthy alvine discharge, which had 

 been prepared 96 hours previously, were injected into its right femoral vein. The 

 dog rapidly recovered, and seemed to be but little afl"ected. 



Presently, it appeared to become drowsy, and in the cours 3 of half an hour symptoms 

 of great irritation of the bowels were manifested. The animal was evidently much 

 griped, and passed several mucous stools mixed with blood. This it continued to do 

 during the day, numerous gelatinous flocculi also being mixed with the dejections. 

 The flocculi when subjected to microscopic examination consisted of exudation cells 

 (similar to those occurring in the flocculi of cholera dejecta), together with a few 

 epithelial cells and structureless gelatinous material. 



Thirteen hours after the operation the dog died, and a post-^mortem examination 

 was made immediately. No fluid in peritoneum, no evidence of peritonitis ; mesenteric 

 glands much enlarged : dark pink internally and containing fluid of a similar colour. 

 The small intestines were very pale externally, whereas the mucous surface was of a 

 dark pink colour, being coated with a reddish mucous substance, which on removal 

 showed the epithelial coat unaffected, and the mucous membrane not congested. No 

 further evidence of morbid change could be discovered. 



