1 1 4 Researches Regarding Cholera : The Blood. [part i, 



killed in order to note the condition of the viscera. No lesion whatever could be 

 found. 



A drop of blood was carefully removed from the right external iliac vein and 

 placed in a wax-cell, and a drop of fluid from the interior of a mesenteric gland was 

 similarly enclosed for observation. During the three days that they were thus 

 watched, not a single distinct monad nor bacterium was seen in either of the speci- 

 mens. 



Experiment LXIV. — A large pariah dog was placed under chloroform, and an 

 ounce of the same solution as used in Exp. LVIII, etc., 100 hours old, was 

 injected into the right femoral vein. It continued drowsy for some time, vomited 

 a large quantity of bilious matter, and by the next day was tolerably well. The 

 wound, however, had assumed an unhealthy, sloughy appearance, so the animal 

 was killed forthwith. There was no peritonitis, the intestines were normal in every 

 way, so were all the other viscera, thoracic and abdominal. The bladder was full. 



A wax-cell preparation of a drop of blood removed from the external iliac vein 

 of the unwounded side, and a similar preparation of fluid pressed out of a mesenteric 

 gland, were kept under observation for three days, during which period neither 

 monads nor bacteria were seen in the former, but an abundance of white cells, whereas 

 in the latter a few bacteria eventually appeared. 



Experiment LXV. — A large healthy pariah dog was placed under chloroform, and 

 five drachms of precisely the same fluid as used in the last experiment were injected 

 into its left femoral vein. After the operation it seemed to be much depressed, and 

 vomited several times. The animal continued in this condition for two and a half 

 hours, when it died. 



A 'post-mortem examination was immediately made, and it was found that the 

 small intestines, though very pale externally, were internally deeply congested, and 

 the lumen of the gut choked with a semi-fluid slimy substance, consisting chiefly of 

 detached epithelium, the individual cells being in a perfect state of preservation. 

 Beneath this substance the villi were seen to be deeply congested, presenting a 

 brush-like appearance. The stomach was healthy, and so was the large intestine. 

 The mesenteric glands looked healthy, and so did the remainder of the abdominal 

 viscera. There was no peritonitis nor pleuritis, but there seemed to be some slight 

 pericarditis. The lungs were collapsed and pale, and both sides of the heart contained 

 fluid blood. 



(/) — The injected material beinyj five days old. 



Experiment LXVI. — A powerful pariah dog was placed under chloroform, and 

 half an ounce of the decomposing solution of foecal matter used in Exp. LTV, 



