1 1 8 Researches Regarding Cholera. [part i. 



cavities full, but not distended with fluid blood, and the auricle and ventricle 

 empty. 



A preparation of blood from the right side of the heart was mounted in a 

 wax-cell and set aside for further examination, along with the preparation procured 

 previous to the operation. Both preparations were examined on the following morning 

 at 9 A.M., when the one had been kept for above twenty-four and the other for 

 about fourteen hours. On examination the former was found to be quite free from 

 bacteria, and to contain very abundant bioplasts in the pus-like condition previously 

 alluded to, whilst the latter only differed from it in there being rather fewer bioplasts 

 free in the serum. They were again carefully examined two days subsequently. 

 They both remained very fluid in consistence, very few bioplasts were present in 

 a distinctly defined condition, they were aggregated in masses, and appeared to be 

 rapidly undergoing disintegration. Neither bacteria nor active molecules could be 

 anywhere detected in either preparation. On subsequent examination the only 

 changes observed in either specimen were gradual disintegration, and there was no 

 development of bacteria in them. 



Experiment LXIX. — At 12 noon of the same day in which the preceding 

 experiment was performed, chloroform was administered to another large healthy 

 pariah dog, and about one ounce of the same choleraic material was injected into 

 the peritoneal cavity through a canula. The dog soon recovered from the primary 

 effects of the operation, and was not affected by such distinctly marked rigors as the 

 former animal. 



His general demeanour, however, was precisely similar to that observed as the 

 result of the other operation ; he was profoundly depressed, lying perfectly quiet and 

 neither whining nor showing any other indication of pain. Neither fceces nor urine 

 were passed throughout the whole day, and there were no evidences of spasm of any 

 of the muscles. As he appeared fully less depressed than the other dog, it was 

 decided to allow him the chance of surviving until the next morning, and his 

 condition at that time had not materially altered, the only change perceptible being 

 an apparent increase in the depression. 



Chloroform was, accordingly, again administered at 7-30 a.m., 19| hours after the 

 operation, and the administration carried on until respiration ceased. 



The abdomen was opened before complete cessation of respiration and circulation. 

 The cavity was distended with fluid of a somewhat less marked sanguineous hue than 

 that present in the previous experiment, but, otherwise, it presented the same 

 characters both to the unaided eye and when subjected to microscopic examination. 

 The peritoneum, both visceral and parietal, presented well marked signs of inflam- 

 mation, and the vessels on the intestines were highly injected and very red. 



There were a few shreds of soft lymph diffused over the surface of the liver, 

 which, on microscopic examination, were found to consist of aggregations of more or 



