PART I.] Effects of Injecting Red Serozis Peritoneal Fluid. 1 2 1 



Experiment LXXII. — An extremely powerful pariah dog was, with much difficulty, 

 brought under the influence of chloroform at 8 a.m., and about six drachms of a de- 

 composing solution of beef were injected into the peritoneal cavity. The solution had 

 been prepared ninety-six hours previously, and at the time of the injection was of an 

 intensely fetid odour and swarming with active bacteria and short vibriones. 



The dog was somewhat dull and depressed throughout the course of the day, but 

 on the following morning he did not appear to have become any worse, and was still 

 so strong, as to give much trouble whilst chloroform was again administered until the 

 cessation of respiration. 



A post-mortem examination was then performed at once. 24 hours after the 

 injection of the fluid had taken place. On opening the abdomen the cavity was 

 found to contain about two ounces of red serous fluid almost resembling pure blood in 

 appearance. A preparation of this fluid was mounted in a wax-cell and examined one 

 hour afterwards. It was then found to contain numerous red blood-corpuscles and 

 myriads of active amoeboid bioplasts, but no bacteria could be detected in it although 

 they were specially searched for under a ^th immersion lens. 



On the following day, however, there were plenty of active bacteria present. The 

 red corpuscles remained unchanged ; but the bioplasts had lost all amoeboid motion, 

 had assumed a more or less spherical form, and were in process of disintegration. 

 By the following morning the activity of the bacteria had ceased, and shortly after- 

 wards the preparation dried up. 



The intestines were congested externally, and their internal surface presented 

 patches of the tarry sanguineous effusion described as occurring in the preceding 

 Experiments Nos. LXVIII, LXIX, etc. The appendix vermiformis was of very large size, 

 but appeared to be quite healthy. The rest of the abdominal and thoracic viscera 

 appeared not to be affected in any way, and the bladder was full of urine. 



A specimen of blood from the right ventricle was mounted in a wax-cell and 

 examined an hour afterwards. No monads or bacteria could be detected in it, but an 

 abundance of delicate, active white corpuscles were present. The preparation was kept 

 under observation for some days, but there was no indication of the development of 

 bacteria in it. 



Experiment LXXII 1. — A healthy pariah dog was put under the influence of 

 chloroform, and four drachms of the fluid from the peritoneal cavity of the dog of 

 Experiment No. liXXII were injected into the abdomen. The fluid had been obtained 

 at the post-mortem examination in the above experiment, which was performed im- 

 mediately before the injection took place. 



The animal rapidly recovered from the influence of the chloroform, and appeared 

 to be little affected by the operation. It remained quiet during the day, showing nO 

 symptoms of pain, or of cramps, but it vomited once or twice. Death occurred during 



