126 Researches Regarding Cholera. [parti. 



evening, and on the following morning, 24 hours after the operation, it appeared to. 

 be perfectly well. It was, accordingly, again put under chloroform and the abdominal 

 cavity opened. There was considerable inflammation of the parietal peritoneum, the 

 mesentery and intestines were intensely injected and inflamed, but there was no fluid 

 present. 



A loop of the small intestine was ligatured, the ligatured portion filled with 

 tepid water, by means of a pointed syringe introduced through the walls, and the 

 gut returned to the abdomen, which was then sewn up. The administration of 

 chloroform was then continued, and the animal died under its influence after an 

 hour. 



A post-mortem examination was performed about half an hour after death. The 

 interior surface of the small intestines with the exception of the ligatured loop was 

 intensely congested, the latter portion had lost all traces of congestion, presented a 

 macerated appearance, and was covered with a layer of soft pale loose epithelium. No 

 other changes observed. 



Experiment LXXIX. — A healthy pariah dog was put under the influence of 

 chloroform, and one ounce of a choleraic evacuation, which had been passed by a 

 patient in hospital a few minutes previously, was injected into the peritoneal cavity. 

 The fluid employed was the same as that used in Experiment I of the series of 

 injections into the veins, and the two operations were performed at the same 

 time. 



The animal appeared to be very little affected by the operation, continued in the 

 same condition throughout the course of the day, and on the following morning, 

 twenty-four hours after the injection, seemed to be quite well. 



It was, accordingly, again put under the influence of chloroform and the 

 abdominal cavity opened. It contained an abundance of yellowish watery fluid, 

 which, on microscopic examination, was found to be full of exudation-cells and 

 perfectly free from bacteria. The parietal peritoneum was densely injected and showed 

 distinct evidences of healing peritonitis. 



The small intestine was deeply congested. A loop of it having been ligatured, 

 a solution of salt and water was injected into the ligatured portion. The intestine 

 was then returned and the abdomen closed. 



The administration of chloroform was continued, and the animal killed at the 

 close of an hour. The ligatured loop of intestine was found to be full of fluid, no 

 absorption appearing to have occurred. The interior surface was coated with white 

 gelatinous matter, and flocculi of a similar nature were floating in the fluid. 



These flocculi were found, on microscopic examination, to be mainly composed of 

 epithelium, and, when the gelatinous coating was scraped off, the mucous membrane 

 beneath it was found to be deeply congested. The rest of the intestine was intensely 

 congested, and showed patches of the prune-juice coating so characteristic of the 



