1 76 Researches Regarding Cholera : the Blood. [part 1. 



Series I. — Ex-periments illustrating the effect of Heat on the Virus of a GoJ)ra. 



Experiment 1. — Injected 20 minims of the diluted virus (24 hours old) by means 

 of a sub-cutaneous syringe into the thigh of a fowl. Died in 

 13 minutes 10 seconds. 

 Experiment 2. — Injected 20 minims of the diluted poison, previously heated to 

 212° F., into the thigh of a fowl — the poison 24 hours old. Died 

 in 32 minutes 45 seconds. The symptoms in both cases were 

 precisely the same. 

 Experiment 3. — Injected 20 minims of the diluted virus, not heated (48 hours old), 



into the thigh of a fowl. Died in 12 minutes 15 seconds. 

 Experiment 4. — Injected 20 minims of the diluted virus, heated to 212° (48 hours 

 old), into the thigh of a fowl. Died in 44 minutes. 

 The solutions of the virus used in Exp. 3 and 4, having become 48 

 hours old, contained active bacteria ; the boiled sample, however, 

 contained far more than the unboiled. 

 All that remained of the virus used in the four foregoing experiments, now 

 seventy-six hours old, was put into a single test tube and still further diluted with 

 distilled water. The tube was then partially submerged in hot water in a closed vessel 

 and thoroughly boiled for ten minutes. The boiled solution exhaled a very offensive 

 odour, something like putrid fish. This was filtered, and the clear fluid obtained used 

 in the following experiment : — 



Experiment 5. — 20 minims of the above boiled and filtered solution of snake- 

 venom were injected into the thigh of a strong fowl. The 

 animal became drowsy in the course of half-an-hour, and sub- 

 sequently presented all the symptoms manifested by the former 

 animals, only in a milder degree. Died in 4 hours 43 minutes. 



Series II. — Ex'perinients illustrating the effect of Heat on the Virus of an 



Australian Snake. 



Experiment 1. — Injected 15 minims of the unheated and unfiltered solution of 



the virus (quite fresh) into the thigh of a fowl. Died in 2 



hours 16 minutes. 

 Experiment 2. — Injected 20 minims of a filtered solution of fresh virus (which 



had been heated to 212° F. as in Series I) into the thigh of 



a fowl. Died in 2 hours 23 minutes. 

 Experiment 3. — Injected 20 minims of the unheated solution of the virus (24 



hours old) into the thigh of a fowl. 

 Experiment 4. — The same as Exp. 3. — Both fowls died within 24 minutes. 



