PART II.] Cultivation- Experiments with the Morbid Products. 377 



As time went on, this pink staining continued gradually to increase in intensity, and 

 ultimately the deposit became entirely of a dull brick-red mingled with patches of 

 rosy pink. 



The most marked changes observed by aid of the microscope consisted in a great 

 increase in the amount of mycelial filaments in the deposit. These were found in special 

 abundance in the flocculent patches adhering to the sides of the bottle, and where 

 they were present in abundance the brightest rosy colour also generally prevailed. Among 

 and attached to the filaments, in many places, numerous large cyst-like bodies were 

 found on carefully teasing out the flocculi (Plate XXVI, Fig. 7). These were rounded, 

 of diameters ranging on an average from -^\-^' to -i^", and in many cases were full of 

 roundish or oval spore-like bodies of considerable size. In colour, like the filaments 

 with which they were connected, they varied greatly ; for, while many were colourless, 

 or exhibited various shades of buff or yellowish, others were of a bright pink or rosy 

 hue. They frequently showed traces of a cellular structure, more or less distinctly. 

 These could, in general, be made out readily by examining the cysts in rather deep 

 focus, so as to bring the profile of their broadest portions into view. The constituent 

 cells of the walls were then clearly brought out, giving rise to an appearance of 

 a looped double outline bounding the mass of the cyst. The cellular structure was 

 also seen to advantage in many cases where rupture of the cyst had occurred, with 

 more or less complete evacuation of the contents. The latter were like their envelopes 

 frequently stained of a pink colour. The precise nature of the connection of the cysts 

 to the filaments, and their mode of development, conld not be thoroughly ascertained, 

 as they were so closely entangled among the meshes and covered by the ramifications 

 of the mycelium as to render it a matter of great difficulty to free them for examination, 

 but it was clearly ascertained in several instances that an organic connection existed 

 between them. 



The nature of these bodies was for some time a matter of great doubt and obscurity, 

 but they were ultimately ascertained to be imperfectly developed Eurotia of the common 

 yellow Aspergillus growing on the sides of the bottle and surface of the fluid. Some 

 of them having been observed in many respects very closely to resemble in structure 

 and form the eurotial structures, which we had frequently obtained on the mycelium 

 of Aspergillus when submerged or grown on very moist substrata, suggested the 

 renewed examination of the mould on the surface of the water and sides of the bottle 

 — above the fluid in this instance. On doing so, no doubt could remain as to the 

 nature of the submerged bodies. Some of the patches of mould on the sides of the 

 bottle, and which extended from above downwards into the fluid, showed normal yellow 

 specimens of the Eurotium of the common yellow Aspergillus in their upper portions, 

 and a series of transition forms lower down, until in the submerged parts specimens 

 were present which were precisely similar to the cysts of the deposit, save that none 

 of them were of a pink colour, but all colourless or pale yellow (Plate XXVI, Fig. 6). As 

 however the presence and degree of colouring in the cysts below was not a uniform 



