PART II.] Cultivation- Experifnents with the Morbid Products. 379 



A portion of the cartilage was removed and set in a moist chamber for further 

 examination. Some of the perithecia assumed a yellowish tint, but the majority 

 remained unchanged, and the principal growth observed occurred in the mycelium. 

 The filaments of this became greatly developed, ramifying and anastomosing over the 

 cartilage and forming closely adherent networks over the surfaces of the perithecia. 

 They presently gave origin to an abundance of erect filaments bearing the ordinary 

 fructification of Aspergillus. In many instances these filaments appeared to arise 

 directly from the perithecia, but this was apparently due rather to their origin from 

 adherent mycelial filaments than to the germination of the spores in the interior of 

 the perithecia, or any outgrowth from their walls. The heads of the Aspergillus were 

 at first white, and ultimately assumed the bright green tint characteristic of Aspergillus 

 glaucus. Spores which had escaped from ruptured perithecia also quickly germinated, 

 and the specimen rapidly became so obscured by a dense growth of mycelium and 

 fructification as to be no longer fit for examination. 



Various other macerations of the morbid products of the ochroid variety of the 

 disease were kept under observation during various periods, but in none of them did 

 a development occur as in the case described, nor were any special organisms observed 

 to occur in connection with them which did not equally occur in macerations or other 

 cultivations of other substrata. 



G. — Cultivations in which the morbid products of the pale variety had been 

 intentionally inoculated with various spores, etc. 



Another series of cultivations was conducted with similar materials, but in which 

 these were intentionally inoculated with the conidia and mycelia of various species 

 of fungi. The following may serve as an example of such experiments and of the 

 results occurring in them. 



Cultivation VI. — Cultivation of inoculated materials. A mass of roe-like bodies, 

 collected from the cavities in Specimen No. Ill (page 349) of the present report, 

 were immersed in water for several days, the fluid being occasionally changed in 

 order to remove the spirit. It was then set in a moist chamber, and inoculated 

 with some of the black-capsuled Mucor and brown and yellow Aspergilli, previously 

 described as occurring abundantly in some of the other cultivations. The fungi 

 rapidly grew and spread over the substratum, covering it with a thick crust 

 principally composed of the fructification of the Aspergilli — -the brown species 

 occurring in considerable excess of the yellow one. 



A month after the inoculation had been performed, this crust was broken up 

 and a layer of bright red matter, varying from rosy pink to strong carmine in colour, 

 was found beneath it on the surface of the substratum. On microscopic examination, 

 this coloured layer was found to be due to a diffused staining of the substratum where 

 the mycelium had penetrated it. Where this had occurred, the material was also 



