FART III.] Artificial Cultivation- Experiments of Virus. 583 



on a glass slide in the usual manner and kept at temperatures ranging from 32° to 

 39° C. for a day or two ; then a portion of the cultivated substance was transferred to 

 a second slide with fresh aqueous humor, and so on, till from a third to an eighth 

 generation was reached. With material thus obtained seven animals were inoculated 

 at different stages of the cultivations. All the animals are described as having been 

 affected, but it would appear that death did not result. Doubtless further information 

 as to the symptoms, etc., manifested by the inoculated pigs will be furnished when 

 full details of the experiments are published. In the meantime it may, however, be 

 noted that it is not mentioned that bacilli were found in the blood of the inoculated 

 animals. 



Dr. Klein states that the cultivated liquids proved, on microscopic examination, 

 to be "the seat of the growth and development of a kind of bacterium which has all 



Fig. 46. Fig. 47. Fig. 48. 



Fig. 46.— The Bacillus of infectious Pnevmo-enteriti^ of the pig, cultivated in aqueous humor of 



rabbit, showing spores germinating into rods, isolated rods, and series of rods. 

 Fig. 47.— From a similar specimen, as in Fig. 46, at a later stage ; most of the rods have grown into 



long filaments. 

 Fig. 48.— Showing the formation of bright cylindrical spores in the filaments at a later stage 



The drawings are represented as the objects appear when seen under a Zeiss's F. objective, 

 and Hartnack's III eye-piece, fitted to a Hartuack's small stand. (After Klein.) 



the characters of Bacillus sitbtilis (Cohn)"— a figure of which, copied from Cohn's 

 paper, will be found on another page (Fig. 49). The rods of the ing-bacillus (Fig. 46) 

 are referred to as being thinner than those described by Cohn as occurring in hay 

 solutions, also thinner than those of the Bacillus anthracis, and, unlike the latter 

 (according to Davaine, Pasteur, Koch, and others) possess a moving stage.* It will, 

 however, be recollected that Dr. Ewart has shown that Bacillus anthracis may also 

 manifest very active movements. Under favourable circumstances the filaments grow 

 into leptothrix-like filaments (Fig. 47) just as other bacilli are known to do. 



"In these filaments," writes Dr. Klein, "highly refractive spores make their 

 appearance (Fig. 48). These become free after the disintegration of the original 



* The letters A, B, used in the original figures (as given in the Microscopical Journal) appear to have become 

 accidentally transposed by the lithographer, as what is referred to in the text under " A, Bacillus of. infectious 

 Pneimo-enteritis of the pig, cultivated in aquemn humor, showing spores germinating into rods, isolated rods 

 and series of rods," evidently refers to B in the plate, and not to the figure marked A. 



