6.3,4 ^^ Flagellated Organisms in the Blood of Animals. [part hi. 



woodcut (63) ; a vacuole-like spot is observable at one part, and the parasite is granular 

 almost along its entire length. Near this specimen a curved protoplasmic object 

 was observed to alter its form very slowly, as shown at «,, 6, c. Its further changes 

 could not he followed, as it was lost whilst its form was being outlined, but I have 

 on two or three occasions observed objects of this character associated with these 

 parasites, and sometimes think that they must represent either an earlier or a later 

 stage of them than is ordinarily seen. 



Further specimens of blood were obtained from the puppy on the 29th and 

 30th of October, but no organisms could be detected ; on the 3rd November 

 however, it is noted that the organisms were very numerous in the blood, and 

 that " the dog looks remarkably well." 



Shortly afterwards the puppy was taken to Calcutta, and when examined on the 

 25th November no organisms could be detected in its blood. On the 3rd December, 



Fig. 64. — a — d appearances presented by one of the flagellated organisms which had apjilied itself 

 to a red blood-corpuscle, x 1000 diameters. 



however, they were readily found. A specimen which was observed on this occasion 

 may serve to illustrate a phenomenon which I have frequently observed in connection 

 with like organisms in the rat. A slide of blood which had been kept in a moist 

 chamber for twenty-four hours having been placed under the microscope, the eye was 

 attracted by the way in which one of the parasites appeared to play with a red 

 blood-corpuscle. It was watched for fully an hour, until, in fact, the field was 

 disturbed by the evaporation along the edge of the cover-glass. Its movements 

 were sluggish and just sufficient to slightly shift the corpuscle. It had not 

 attached itself to the corpuscle by either of its ends, but at a spot about 8 //, from 

 the point of the thicker end as shown in Fig. 64, a to d. Sometimes there appeared 

 to be a slight interval between the corpuscle and the parasite (Fig. 64, a), and 

 occasionally even a greater interval than is indicated in the woodcut, but both 

 parasite and corpuscle, nevertheless, continued to move in unison, as though some 

 filamentous connection existed between them, which, however, was too delicate 



