of. 



the first cleavage 

 icus scores. germinated in u**Tlateral have failed so far 



^L^' _^^ A 



All attempts to solve thi groblera as to wh^tr"wave lengths of 



;ht are responsible lor the orientation 



\ 

 plane o 



^^ ^ 



and apparently -aTnvalsucceed^fith the pparatus described 



in the first port of th^>^pape>>*41owever it seems worth while 

 to report t-e metlpyrfs used and why theyhmte failed. T 7ork v/ill be 

 continued on^fenis investigation at the earliest o*pXjrtunity, PS it 

 is mereiar a matter of obtaining the right conditions of "gmjwth 



bejtffnd the light filters, together with a sufficiently strong 



illumination. 



The fruiting plants of Fucus eyanescens were collected at 

 Sausilito at low tide of one day and kept over night in damp news 1 *' 



papers. The next mornibg they were dried slightly by exposing them 

 to the air for about half an hour or less. The fruiting tips were 

 then cut off a'^ submerged in *ta- v/ater in the culture dishes. After 

 fifteen minutes many eggsjand sperms have settled to the bottom of 

 the dish or can be scraped 6ff into the water. The piece of plant is 



then removedVnd the dish is placed in the rack which fits into 

 a dark box behind a filter screen. Each box with its cultures 



is placed at the proper distance from the light so that each is il 

 luminated with equal intensity. These distances for each 



the raethod by which they -'ere obtfii- ed^ 



by five as the case might be 



distance given tnei? however was divided by four or 



; / *\ 



in order to increase the intensity of the illumination equally 



for all. The illumination with the electric arc was continued 



V ^ 



for eight in an otherwise darkened room, the carbons being ad- 



justed every five minutes. This time was judged more than sufficient 



M*4 



to produce **jseffect since it was found in Cystoseira ( 



