



METHODS 

 THF; CULTURE MBDTUi 



The corn meal apar, employed in these experiments 

 was prepared according to the procedure described "by Shea>- 

 and Wood using 20 g. of corn meal and 15 £. of agar shreds 

 for each liter of water. More water was added "before the 

 final filtering so that there wae one liter of the medium for 

 each 20 g. of corn meal originally used. 



The exact chemical and physical nature of such a cul- 

 ture medium cannot of course he stated. It undoubtedly con- 

 tains a large number of inorganic salts and a still larger 

 number of organic compounds, all in rather low concentration. 

 It also contains various substances in a state of suspension 

 and, being a gel there is no tendency for these to precipi- 

 tate out. Since the whole problem of definite media for 

 fungus and bacterial cultures remains for the most part entire- 

 ly untouched, especially for cases where so-called solid media 

 (with agar, gelatine, etc.) are employed, and since the time 

 available for this study was very limited, it was decided to 

 make no attempt to devise a nutrient medium of known compo- 

 sition ( which would be a very difficult undertaking in the 



present state cf our knowledge) nor even to find out what any 



Shear, C. L. , and Weed, Arna K. , Studies of fungus parasites 

 belonging to the genus Grlomella. Bui. 252, Bureau of Plant 

 Ind. U. S. D. A 1912. 



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