52 JOHNE^S DISEASE 



obtaining positive reactions with a specific diagnostic 

 vaccine — the following condition was observed post 

 mortem : The animals were somewhat wasted, and 

 showed enlarged glands throughout the abdominal 

 cavity and thorax. When opened, the glands showed 

 an oedematous condition, and some pigmentation most 

 marked in the medulla. 



In the animal inoculated intravenously the intestines 

 were distinctly thickened, especially in the vicinity of 

 the ileo-caecal valve; but in the animal inoculated 

 intraperitoneally there was no evidence of thickening. 



Both cases showed only a few bacilli in the lesions, 

 and none were found in the thoracic glands. 



The condition of these sheep was identical with that 

 observed in the experimentally inoculated calves which 

 w^ere killed before the disease had become advanced. 



The liver in the sheep appeared to be fatty, and 

 presented the same appearance as that sometimes met 

 with in cattle. 



M'Fadyean, in his Annual Report of the Royal 

 Veterinary College, published in the Journal of the 

 Royal Agricultural Society^ 1907, wrote : 



**.... In ascertaining the cause of chronic 

 diarrhoea among deer kept in a park, and when 

 one of the diseased animals was killed the post- 

 mortem examination unexpectedly disclosed the 

 fact that it was the subject of Johne's disease. 

 The fact that deer may suffer from the disease 

 is not one of much direct interest, but it is of 

 importance as suggesting that other ruminants, 

 such as the sheep, may not be immune." 



In July, 191 1, the present authors inoculated two 

 goats with pure cultures of Johne's bacillus. Goat i 

 received intravenously 3 c.c. of an emulsion of bacilli 



