66 JOHNE^S DISEASE 



cultures, which were isolated on glycerine-serum^ 

 glycerine-agar, and other media, some of which con- 

 tained decoctions of the grass Phlemn p7^ate7ise, were 

 submitted to Koch and Rabinowitsch, who declared 

 them to be avian tubercle bacilli. Inoculation experi- 

 ments with these cultures failed to produce Johne's 

 disease, but caused lesions in the small laboratory 

 animals which proved them to be the avian type of 

 tubercle bacillus. Mettam, in a private communication, 

 states that he has also isolated a culture of the avian 

 tubercle bacillus from a case of Johne's disease. We 

 consider these results to be secondary infections with 

 the avian tubercle bacillus. This view is supported 

 by a large number of authorities, including Bang, 

 Miessner, MTadyean, Markus, and Meyer, who, pre- 

 vious to the year 191 1, experimented with infected 

 material from cows, and failed to obtain cultures of the 

 specific bacillus. It will be shown, also, that when the 

 bacillus has been isolated on the special medium, it 

 can be subcultured on ordinary laboratory media only 

 after prolonged cultivation and acclimatization outside 

 the animal body, which proves definitely that the 

 bacillus is not a variety of the avian tubercle bacillus. 

 In 1908, Bugge and Albien published a short note to 

 the effect that they had succeeded in obtaining a pure 

 culture of Johne's bacillus {Paratuberkelbazillen) from 

 the mesenteric gland of an affected animal. No par- 

 ticulars as to the medium were given in this paper; 

 but in 1910, Albien published a further note giving the 

 constitution of the medium used. This was as follows : 



Nahrstoff Heyden 5-10 



Agar I0-20 



Glycerin 20-30 



Kochsalz ^ 



NormallQsung von Kristallsoda (28 : 100) ... 5 



Aqua dest 1,000 



