72 JOHxNE'S DISEASE 



tubercle bacillus medium then prepared, only one of 

 these tubes was subcultured. This one was made from 

 a gland. Films from these subcultures were examined 

 microscopically at intervals of about four or five days. 

 After nineteen days the subculture on the special 

 medium showed quite definite evidence of multiplica- 

 tion ; the bacilli had grown larger and thicker ; they 

 were well stained, and were present in large, close 

 masses. Subcultures were made from this tube on 

 to fresh tubes of various media, including one tube of 

 the special tubercle bacillus medium. These were ex- 

 amined at intervals as before, and the subculture on 

 the special medium showed microscopic evidence of 

 growth in ten days. Both the first and second sub- 

 cultures showed growth visible to the naked eye after 

 four weeks, and this gradually increased, reaching a 

 maximum in about eight weeks. 



These tubes were easily subcultured on to fresh 

 tubes of the same medium, but on none of the ordinary 

 laboratory media were we able to get any evidence of 

 growth. 



The third case of pseudo-tuberculous enteritis was 

 obtained from Mr. Hamilton. Specimens of intestine, 

 but no glands, were received on September 23, 1910. 

 They showed the typical lesions of the disease, and 

 a very large number of Johne's bacilli were present 

 in the tissues. When delivered, the specimens had 

 already commenced to decompose; but from them 

 cultures were made as previously described, both 

 directly, and indirectly after treatment with ericolin 

 solution on various media, including tubes of the 

 special tubercle bacillus medium. The tubes were 

 capped with gutta-percha tissue, and placed in an 

 incubator at 39° to 40° C. The results were the same 

 as in Case 2 ; all the direct tubes were badly contami- 



