CULTIVATION OF THE BACILLUS 73 



nated, and those tubes which had been inoculated 

 with material previously treated with ericolin solution 

 grew only a few contaminating colonies. Of the 

 latter the cultures on the tubes of special medium 

 were subcultured from uncontaminated areas on to a 

 number of fresh tubes of various media, including the 

 special medium. The subcultures on the ordinary 

 media remained sterile, but those on the tubercle 

 bacillus medium grew Johne's bacillus in pure growth, 

 and were without difficulty subcultured on to fresh 

 tubes of the special medium. Naked-eye evidence of 

 growth was present in the first subcultures after about 

 six weeks. 



The fourth case was obtained from Mr. De Vine, a 

 specimen of the intestine being received at the In- 

 stitution on January 26, 191 1. It showed the typical 

 lesions of pseudo-tuberculous enteritis, and a large 

 number of the specific bacilli were present in the 

 lesions. Since the specimen was quite fresh, cultures 

 were made, as previously described, from the ileum, 

 caecum, and ileo-caecal valve, directly on to nine tubes 

 of the special tubercle bacillus medium ; these were 

 capped, and placed at 39° to 40° C. After three weeks' 

 incubation two tubes were found to be contaminated, 

 whilst the remainder were covered with extremely 

 minute colonies of Johne's bacillus, and showed no 

 contaminations. The cultures grew well, and were 

 subcultured without any diflficulty on to the special 

 medium. Subcultures made on to Dorset's egg 

 medium, glycerine-agar, and various other media gave 

 no growth. 



Case 5 was obtained from Mr. Hamilton, and was 

 received at the Institution on February 8, 191 1. The 

 specimen, consisting of ileum and ileo-caecal valve, 

 showed the typical lesions of pseudo-tuberculous 



