NATURE OF THE "ESSENTIAL SUBSTANCE'' 97 



Besides these we tested an extract of beef, to portions 

 of which were added one of the following substances 

 in the following quantities : 



Glycerine 



Glycerine 



Glycerine 



Glycerine 



Mannite 



Mannite 



Erythrite 



Erythrite 



Dulcite 



Methyl alcohol 



Methyl alcohol 



Absolute alcohol ... 



Absolute alcohol ... 



Propyl alcohol 



Isopropyl alcohol ... 



Per 

 Cent. 



I 

 2 



4 



lO 



I 



2 

 I 



2 



I 

 2 



5 



2 



5 



I 

 I 



Per 



Cent. 



Tertiary butyl alcohol 



Glucose 



Dextrose 



Laevulose 



Lactose 



Maltose 



Saccharose 



Rhamnose 



Galactose 



Raffinose 



Potassium sodium tar- 

 trate , 



Amygdahn , 



Asparagin 



Glycol 



To all, except the Naegeli media, ^ per cent, of sodium chloride 

 was also added. 



B. phlei was grown on flasks of all the media, many 

 of which were also used for growing the "nasen- 

 schleim" bacillus and Grassberger's bacillus. The 

 quantity of growth obtained with B. phlei varied 

 considerably, the best being on the broth containing 

 glycerine; but growth was also fairly good on the 

 media containing mannite, erythrite, glucose, dextrose, 

 laevulose, 2 per cent, absolute alcohol, or 2 per cent, 

 propyl alcohol, and also on Gordon's fluid + glycerine 

 and on the hay extract. In the case of the " nasen- 

 schleim " bacillus and Grassberger's bacillus much the 

 same results were obtained, and in every case the 

 bacilli had formed the essential substance — that is, 

 when killed and dried and added to egg^ they made 

 good media for Johne's bacillus. The media, however, 

 varied, and although space will not allow us to give 

 details of the many hundreds of experiments carried 



7 



