112 JOHNE'S DISEASE 



positive and two negative reactions. When killed it 

 showed the characteristic lesions of the disease. 



In his paper {Norsk Veterincer-Tidsskrift, No. 8, 

 August, 191 1), Malm gives the following notes on the 

 two calves : 



CALF A. 



3.4.08. Calf fourteen days old, fed with infected intestine in milk. 



14.5.08. Again fed from another animal. No fever, no diarrhoea, 



no bacilli in faeces till 23.6.09. 



10.3.09. 2 c.c. avian tuberculin ; well-marked reaction — 38*8 to 40*9. 



No diarrhoea, no fever next day. 



2.4.09. ^ gramme Old Tuberculin ; negative reaction. 

 5.4.09. Uncertain reaction (ophthalmic) with avian tuberculin. 



13.4.09. Good ophthalmic reaction in other eye. 



23.4.09. I c.c. fish tuberculin + i c.c. blindworm tuberculin sub- 

 cutaneously ; negative reaction. 



17.6.09. Intradermal reaction, avian tuberculin, positive. 



23.6.09. Bacilli in fasces for first time. 



25.6.09. Simultaneous, intradermal, and ophthalmic tests with avian 

 tuberculin. Ophthalmic test, positive. 



28.6.09. Intradermal test, negative. 

 7.7.09. Diarrhoea noticed first time. 0*35 gramme avian tuber- 

 culin ; negative reaction. 



28.9.09. Avian tuberculin ; negative reaction. 

 19. 1 1.09. Diarrhoea again noticed. 



13.12.09. I gramme avian tuberculin ; negative reaction. 



20. 1. 10. Large number of acid-fast bacilli in faeces. 



29. 1. 10. 2 grammes avian tubercuhn ; good reaction (38*5 to 40* 2). 

 31. 1. 10. Killed. All the characteristics of a typical case of Johne*s 



disease. Diagnosis confirmed by B. Bang, then in 



Christiania. 



Scrapings of bowel were inoculated into three 

 rabbits and eight guinea-pigs with negative results. 



CALF B. 



16.4. 10, Fourteen days old. Fed with 150 grammes mucosa of 



bowel of Case 13 in warm milk. 

 2 1.6. 10. Positive reaction to \ gramme of avian tuberculin 



(38-9 to 40-1). Marked diarrhoea after reaction, and 



many acid-fast bacilli in faeces. 

 8.8.10. Old tuberculin ; negative reaction. 



