VACCINES : DIAGNOSTIC AND CURATIVE 118 



27.9.10. ^ gramme avian tuberculin ; positive reaction (39*1 to 40' i). 

 6. 1. 1 1. Same dose of Old Tuberculin ; negative. 



20.1. 1 1. Reacted with avian tuberculin (40'i). 

 1.4. 1 1. No acid-fast bacilli in faeces. 



4.4. 1 1. I gramme of avian tuberculin gave a negative reaction. 



10.4.11. I gramme Old Tuberculin ; negative. 



18.4. 1 1. I gramme Old Tuberculin ; negative. 



10.5.11. Killed. Proved to be a typical case of Johne's disease. 



Miessner and Trapp, using avian tuberculin received 

 from Bang, tested a series of experimentally inoculated 

 animals. Their results are given in the table on 

 p. 114. 



Here the first five calves certainly "reacted," and 

 sufTered an attack of diarrhoea as a result of the 

 disturbance. 



G. P. Male has used avian tuberculin as a test for 

 this condition, the reagent being supplied to him by 

 Stockman. The following are his results : Herd i — 

 Jersey cattle. One bull, ten cows, and eight heifers 

 and calves were tested, and two cows and two heifers 

 gave the following reactions : 



Case (d) was examined by Stockman post mortem, 

 who gave it as his opinion that the animal was affected 

 with Johne's disease, though no acid-fast bacilli were 

 found. None of the animals showed any evidence 

 of tuberculosis post mortem. Herd 2 : Thirty-two 



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