116 JOHNKS DISEASE 



animals had given a negative result when tested with 

 ordinary diagnostic tuberculin. 



In only two of the nine cases detailed above, which 

 Male classed as reactions, did the temperature rise 

 above 104*0° F. Such reactions cannot be considered 

 as very definite, and it is doubtful whether State 

 legislation could be enforced and compulsory slaughter 

 ordered, unless a more marked reaction can be pro- 

 duced. In the case of animals housed specially for the 

 test and unused to being handled, the taking of the 

 rectal temperature and the use of necessary restraint 

 might lead to serious errors. 



Since the publication of Male's results, the present 

 writers have performed some tests with avian tuber- 

 culin. The vaccine in our hands gave negative results, 

 and we concluded that, although some cases of Johne's 

 disease undoubtedly react to large doses of avian 

 tuberculin, the test is very uncertain, and is not of 

 much practical value. Details of these experiments 

 are given at the end of this chapter, where we discuss 

 vaccines prepared from cultures of Johne's bacillus. 



More recently MTadyean, Sheather, and Edwards 

 have published results obtained with avian tuberculin. 

 The test was applied to fourteen bovines, all of which 

 were proved, on post-mortem examination, to be 

 affected with pseudo-tuberculous enteritis. The dose 

 of avian tuberculin given was 8 c.c. ; it was given 

 subcutaneously, and the results are shown in the 

 table on p. 117, which we have compiled from the text 

 of their paper. 



In this table Cases XL and XVI. may be con- 

 sidered positive on the first test, although the reac- 

 tion in Case XI. is not very good. If one were testing 

 with ordinary tuberculin for tuberculosis. Cases VI. 

 and VIII. would be classed as doubtful. 



