128 JOHNE'S DISEASE 



advanced stage, the bacilli being extremely numerous 

 in the films and sections from the diseased gut. Tuber- 

 culosis, which must have been contracted while the 

 animal was in our stables, was present in the lungs 

 and bronchial glands. 



Bovine No. 2 died three weeks after the inoculation 

 (September 17, 1912), and on post-mortem examination 

 showed the typical lesions of Johne's disease. The 

 gut was very congested, and some haemorrhages were 

 present. There was no evidence of tuberculosis. 



Bovine No. 3 died (August 26, 191 2). Post-mortem 

 examination showed advanced Johne's disease in the 

 intestine and mesenteric glands, the bacilli being fairly 

 numerous in films and sections from the diseased gut. 



The five calves tested were those that are also 

 mentioned in Chapter IX., and numbered la, 2a, 3a, 

 4«, and 5a, respectively. They had been inoculated 

 with the strain of Johne's bacillus recovered from calf 

 No. 5 (pp. 123 and 148). The animals did not thrive well, 

 but they showed no clinical manifestations of Johne's 

 disease. All were tested with Vaccine No. 6 about six 

 months after inoculation with the living cultures; 

 calves Nos. 2a and 3a each received 3 c.c, and calves 

 Nos. la, 4a, and 5a each received 5 c.c. The following 

 results were obtained : calves Nos. 2a and 3a showed 

 no rise of temperature in six hours, and through an 

 error were not tested again until twenty-four hours 

 after the inoculation, when the temperatures were 

 normal. The temperature of calf No. la rose to 

 104*6° F. in three hours, that of calf No. 4a to 105° F. 

 in six hours, while calf No. 5a showed no rise in ten 

 hours, but when taken the following morning — twenty- 

 four hours after the inoculation — the temperature 

 registered 105*4° F., and was dropping. 



A few days after the vaccine tests the calves were 



