VACCINES : DIAGNOSTIC AND CURATIVE 129 



killed and post-mortem examinations performed. The 

 results are summarized in the table, and a detailed 

 description of the pathological lesions present is 

 given in Chapter IX., pp. 150 and 151. 



We now tested the vaccine by means of subcutaneous 

 inoculations, and as at the time we had no more 

 bovines at our disposal, we used goats. The goats 

 tested were two which we had inoculated in July, 191 1, 

 with living cultures of Johne's bacillus. These animals 

 are also mentioned (p. 151). 



At the time of testing the vaccine, eleven months 

 after the intravenous inoculation of the living culture, 

 goat No. I was thin, but otherwise there was no mani- 

 festation of the disease. Goat No. 2, which had been 

 inoculated into the peritoneal cavity, was apparently 

 quite healthy. Each animal received 3 c.c. of the 

 vaccine subcutaneously. The temperature of goat No. i 

 rose to io6*4° F. in five hours, reached 106*6° F., and re- 

 mained at 106° F. or over for fully eight hours ; the rise 

 of temperature was accompanied by some diarrhoea. 

 The temperature of goat No. 2 reached a maximum of 

 105*4° F. at the tenth hour, but no diarrhoea ensued. 

 Both animals were killed and post-mortem examina- 

 tions made. Goat No. i showed Johne's disease 

 throughout the intestines and mesenteric glands, and 

 goat No. 2 showed the disease in a very early stage. 

 In both cases the bacilli were found in the diseased 

 tissues, and were isolated on special media (vide 

 table, p. 126). 



Satisfactory results with Vaccine No. 6 having been 

 obtained, a further batch — Vaccine No. 7 — was prepared 

 as follows : We used our strain of Johne's bacillus 

 that had become acclimatized to media containing no 

 other acid-fast bacilli or extracts of such bacilli. It 

 was grown for about four months on broth containing 



9 



