130 JOHNKS DISEASE 



I per cent, peptone, J per cent, sodium chloride, and 

 4 per cent, by volume of glycerine. The cultures were 

 shaken and the large masses of growth allowed to 

 settle. The fluid containing the smaller particles was 

 heated to 65° C. for one hour in a water-bath, and 

 was used without previous filtration. This vaccine 

 was tested on a number of adult bovines. Some were 

 tested at the Brown Institution and others on farms by 

 veterinary surgeons to whom we sent the vaccine. 

 The results are summarized in the table, and we 

 regret that we have been unable in most cases to 

 obtain a post-mortem examination. 



In the table the animals are numbered 4 to 25 

 inclusive, and it is unnecessary to describe each in 

 detail, although the following points may be noted : 

 No. 5, which failed to react to the vaccine, was a very 

 advanced case of Johne's disease. It showed extensive 

 lesions in the ileo-caecal valve, in the lower part of the 

 ileum, and in the caecum. The upper part of the small 

 intestine and the large intestine were less affected. An 

 enormous number of bacilli were present in the mucous 

 and submucous coats of the ileum (see Plates III. 

 and IV.). The abdominal lymphatic glands were also 

 extensively involved, and showed a large number 

 of bacilli. In the opinion of the writers there is no 

 doubt that this case failed to react owing to the very 

 advanced stage of the disease. Cases Nos. 4, 6, and 9, 

 on post-mortem examination, also showed extensive 

 lesions of Johne's disease, but not so marked as in case 

 No. 5. In each case the condition was most evident 

 in the lower part of the ileum and in the ileo-caecal 

 valve. The abdominal lymphatic glands were only 

 moderately involved. In all three cases a considerable 

 number of bacilli were present in the ileum and a fair 

 number in other parts of the intestine and in the 



