VACCINES : DIAGNOSTIC AND CURATIVE 131 



abdominal glands. Case No. 6 also showed tubercu- 

 losis of the bronchial glands. Cases Nos. 12 to 25 

 inclusive were all on the same farm, and were tested 

 by ourselves. The animals were in a bleak, open 

 position, and all were tested on a cold day. It is 

 well known that when tuberculin is used under such 

 conditions the temperature of a tubercular animal may 

 not rise to the same extent that it would under more 

 normal circumstances, and one would expect the same 

 rule to hold good in animals affected with Johne's 

 disease. If this is so, then the temperatures recorded 

 in animals Nos. 12, 13, 23, and 25 all indicate good 

 reactions, and in case No. 15 a fair reaction. It may 

 be noted, too, that the temperature of each animal was 

 subnormal before the vaccine was inoculated, and we 

 were only able to take it at the fourth, sixth, and tenth 

 hours following the inoculation of the vaccine. Case 

 No. 22 showed definite clinical signs of tuberculosis, 

 and gave no reaction with the vaccine. 



More recently we have tested Vaccine No. 7 on five 

 sheep experimentally inoculated. No. i was inoculated 

 by the mouth, Nos. 2 and 3 intravenously, and Nos. 4 

 and 5 intraperitoneally. Ten weeks after inoculation 

 the animals were tested with the vaccine, this being 

 injected subcutaneously. The temperatures were taken 

 hourly after the third hour, up till the eleventh hour 

 following the inoculations, and they were taken again 

 at the twentieth and twenty-fourth hours. 



No. I gave a maximum of io5'o° F. in 8 hours. 

 No. 2 „ „ 105*2° F. in 6 hours. 



No. 3 „ „ 107*1° F. in 6 hours. 



No. 4 „ „ 106*8° F. in 7 hours. 



No. 5 „ „ 107*8° F. in 6 hours. 



It will be observed that in every case the maximum 

 temperature was registered between the sixth and 



