142 JOHNE'S DISEASE 



a cow which was suffering from Johne's disease, but 

 was free from tuberculosis : The milk was centrifuged, 

 and the sediment inoculated into six guinea-pigs and 

 one calf, with negative results in six months. The 

 urine was centrifuged, and the sediment inoculated 

 into six guinea-pigs, which also gave negative results. 

 The mucous membrane of the intestine was also inocu- 

 lated into a number of animals. These are tabulated 

 below, and show negative results with all the animals. 



Six guinea-pigs subcutaneously, negative in four to five months. 

 Six guinea-pigs intra-abdominally, negative in four to five months. 

 Three rabbits subcutaneously, negative in four to five months. 

 Three rabbits intra-abdominally, one died in nine days (peri- 

 tonitis), and the remainder were negative in five months. 

 Six pigeons subcutaneously, negative after five months. 

 One calf intravenously, negative after six months. 

 One calf per os, negative after six months. 

 Three hens per os, negative after four months. 



A large series of inoculations was also carried out 

 with the lymphatic glands attached to the mesentery. 

 These are tabulated belov/, and in all cases show 

 negative results. 



Six guinea-pigs subcutaneously, negative after five months. 



Six guinea-pigs intra-abdominally, negative after two to six 



months. 

 Three rabbits subcutaneously, negative after four months. 

 Three rabbits intra-abdominally, negative after three and a half 



months. 

 Two hens per os, negative after three and a half months. 

 One goat intravenously, negative after eight months. 

 One dog intravenously, negative after three and a half months. 

 One calf intravenously, negative in three months. 

 One calf intravenously, died in two days. 



Negative results were also obtained with the spleen, 

 udder, and lymph glands of the pleura, as shown below. 



Spleen inoculated into four guinea-pigs subcutaneously, negative 

 in four months. 



