146 JOHNKS DISEASE 



tracted Johne's disease as a result of the experiment, 

 and it is to be presumed that the coincident tubercu- 

 losis was an accidental infection contracted by the 

 animal while in the stables. 



Bovine No. 2. — Heifer calf. Previous to inoculation, 

 the temperature of this calf was taken twice daily for 

 twelve days, and was found to be normal for an animal 

 of its age. The faeces were also examined several 

 times for acid-fast bacilli, with negative results. On 

 November 10, 1910, when sixteen days old, the calf 

 was inoculated with a culture of Johne's bacillus. The 

 growth was three weeks old and the third subculture 

 distant from the primary culture from the diseased 

 gland. The growth was washed off with sterile 

 o*8 per cent, sodium chloride, and an opalescent 

 emulsion obtained. This was inoculated into a vein 

 of the left ear, but no immediate symptoms followed. 

 The faeces were frequently examined for the presence 

 of acid-fast bacilli, and from December 15, 1910, to 

 January 17, 191 1, films occasionally showed some 

 thick acid-fast bacilli, which, however, did not resemble 

 Johne's bacillus. These were probably some sapro- 

 phytic " dung bacilli," and from the latter date no 

 acid-fast micro-organisms were found. 



On October 24, 191 1, the animal was killed. On 

 post-mortem examination, the carcass showed no 

 emaciation, and no pathological lesions were found in 

 any of the organs or tissues of the body with the 

 exception of the bronchial lymphatic glands. These 

 were slightly enlarged, and showed several caseating 

 centres in which tubercle bacilli were demonstrated. 

 On microscopical examination, the intestinal lymphatic 

 glands failed to reveal any acid-fast bacilli. Cultures 

 were made in the usual manner from one of the tuber- 

 cular bronchial glands and from beneath the intestinal 



