148 JOHxNE^S DISEASE 



toneal cavity with about 5 milligrammes of a moist 

 growth of Johne's bacillus. This growth was the 

 fourth subculture distant from the primary culture. 

 From the time of inoculation onwards the animal 

 showed no definite symptoms, no acid-fast bacilli 

 could be detected in the faeces, and, with the exception 

 of the periods when various vaccine tests were made, 

 the temperature remained normal. On October 16 

 the calf was killed. The animal was in good condi- 

 tion, and no lesions either of tuberculosis or of Johne's 

 disease could be found. Films made from beneath the 

 intestinal mucosa showed no acid-fast bacilli, and cul- 

 tures from this situation remained sterile, so that the 

 result was entirely negative. 



Bovine No. 5 {Calf No. 5). — This was a red and white 

 male calf, and was received at the Institution January 7, 

 191 1. Repeated examination of the faeces failed to 

 reveal any acid-fast bacilli, and the temperature re- 

 mained normal. On February 21 the calf was inocu- 

 lated intravenously with about 7 milligrammes of a 

 moist culture of Johne's bacillus. The culture used 

 was the fourth subculture distant from the primary 

 growth. No immediate S3^mptoms followed, and there 

 was no rise of temperature. The condition of the calf 

 remained good during March and the beginning of 

 April, but towards the end of the month it became 

 thin and weak. These symptoms increased up to 

 May II, when the calf was not able to rise without 

 assistance, though the appetite was good and the 

 temperature normal. In the early part of June the 

 calf could again walk without assistance, but it re- 

 mained very thin. There was no diarrhoea, nor could 

 any acid-fast bacilli be found in the faeces at any time. 

 On July 12, 191 1, the animal was killed. The carcass 

 showed extreme emaciation, and a small tuberculous 



