150 JOHNE^S DISEASE 



as has been mentioned, was obtained originally from 

 the naturally infected case No. 2 described on p. 71. 

 The culture obtained from the calf was again grown 

 for about six months outside the animal body, and 

 occasionally subcultured. A growth obtained in the 

 third generation, which was about two and a half 

 months old, was then inoculated into five calves. 

 Calves Nos. la and 2a were inoculated intravenously, 

 Nos. 3« and 4a intraperitoneally, and No. s^ subcu- 

 taneously. 



After being tested with various vaccines, these 

 animals were killed a little over six months from the 

 date of inoculation, and, post mortem, all but one 

 proved to be more or less affected (see table, p. 126). 



Calf No. la showed typical lesions of Johne's disease 

 in the intestines and mesenteric glands. The bacilli 

 were most numerous in the tissues of the ileo-caecal 

 valve, but were also present beneath the mucous 

 membrane of other parts of the gut, and in the glands. 

 The animal showed no evidence of tuberculosis. 



Calf No. 2a showed slight lesions of Johne's disease 

 in the intestine and in the mesenteric glands, which 

 were somewhat larger than normal. Several acid-fast 

 bacilli were found in the glands, and a few beneath 

 the mucous membrane near the ileo-caecal valve. No 

 tubercular lesions were found. 



Calf No, 3a showed typical tubercular bronchial 

 glands, but there was no evidence of Johne's disease 

 in the intestine or mesenteric glands. 



Calf No. 4a showed tubercular bronchial glands, 

 which were caseous and in places calcareous. Johne's 

 disease was present in a moderately advanced stage. 

 The bacilli in the intestinal wall were most numerous 

 near the ileo-caecal valve, a situation in which we have 

 usually found the lesions to be most pronounced. The 



