EXPERIMENTS WITH PURE CULTURES 151 



disease in the mesenteric glands was particularly well 

 marked, due, no doubt, to the method of inoculation. 

 Films from these glands showed a fair number of 

 Johne's bacilli in each field. 



Calf No. 5a was small and emaciated. On post- 

 mortem examination it showed typical tubercular 

 bronchial glands and early tuberculosis of the apex 

 of the right lung. There was no definite thickening 

 of the mucous membrane of the intestine, and the 

 mesenteric glands were not much enlarged. Very 

 few acid-fast bacilli were found beneath the intestinal 

 mucosa, but several were present in films from one 

 of the mesenteric glands. 



All five calves showed some congestion of the 

 mucous membrane of the intestines, with occasional 

 haemorrhages. Some of the glands also showed 

 haemorrhages, probably caused by the dose of vaccine 

 which each animal had recently received (p. 128). These 

 conditions were present only in a slight degree in 

 calf No. la. 



From all five cases cultures were made in the usual 

 manner from the intestinal mucosa and abdominal 

 lymphatic glands, and in all cases except No. la the 

 bacillus was recovered in pure culture on a timothy- 

 grass bacillus medium. Tubes of ordinary media 

 inoculated at the same time from the intestine and 

 glands all remained sterile. Thus, out of five calves, 

 four had contracted the disease. 



In July, 191 1, we inoculated two young goats with 

 living cultures of Johne's bacillus. The growth from 

 one tube of medium was made intp an emulsion with 

 10 c.c. of sterile 0-85 per cent, sodium chloride. Goat 

 No. I received 3 c.c. of this emulsion intravenously ; 

 goat No. 2 was given i c.c. in the peritoneal cavity. 



Eleven months later, after being tested with a vaccine 



