152 JOHNE^S DISEASE 



(p. 129), these two goats were killed. At this time 

 one of the animals (goat No. i) was thin, and the 

 inoculation of the vaccine had caused severe diarrhoea ; 

 but in goat No. 2 there were no clinical manifestations 

 of the disease. On post-mortem examination, goat 

 No. I showed typical lesions of Johne's disease 

 throughout the intestine and in the abdominal lym- 

 phatic glands. In films from both places the bacilli 

 were present in fair numbers. Goat No. 2 showed the 

 disease in a very early stage, and several bacilli only 

 were found. From both these cases, however, follow- 

 ing the usual procedure, we were able to isolate the 

 bacilli on the special media (see Plate IX., Figs, i and 2). 



From the positive results obtained in the two goats 

 it is highly probable that these animals, like bovines, 

 can contract the disease naturally, although, as has 

 already been pointed out, Miessner and Trapp 

 obtained negative results with goats that were fed 

 with diseased gut from a cow. 



Quite recently we have reproduced Johne's disease 

 in sheep (Vetermary Record^ Aprils 1913)- Five animals 

 were inoculated with cultures of Johne's bacillus iso- 

 lated from a cow. Sheep No. i was inoculated by the 

 mouth, Nos. 2 and 3 intravenously, and Nos. 4 and 5 

 intraperitoneally. Ten weeks after the inoculations 

 the animals were tested with a diagnostic vaccine. 

 Nos. I and 2 gave doubtful reactions, and Nos. 3, 4, 

 and 5 good reactions {vide Chapter VII., p. 131). 



Shortly after the vaccine tests, animals Nos. 3 and 5 

 were killed and post-mortem examinations made. 

 Both showed definite lesions of Johne's disease, and 

 no evidence of tuberculosis. 



In the case of sheep No. 3, the intestines showed 

 a moderate degree of thickening, most marked in the 

 caecum and ileo-caecal valve, but only a few bacilli 



