DOSAGE OF VACCINES 



221 



hours, according to the resistance of the organism. The stock 

 solution may subsequently be introduced into small sterile glass 

 ampoules of 1-2 c.c. capacity, which, after sealing and standing for 

 twenty -four hours, may again be sterilised for an hour at 60 C. 

 to ensure the destruction of the organisms ; cultures may be made 

 from the sterilised vaccine to ascertain that this is the case. The 

 lower the temperature and the less the heating, consistent with 

 sterilisation, the more active will be the vaccine. 



The annexed Table x gives an idea of the doses of vaccines, their 

 toxicity, and frequency of inoculation. 



The smaller doses are given at the commencement of the treat- 

 ment, and the doses are gradually increased. 



The writer has employed endotoxin solutions as vaccines and 

 believes they are very efficient. 



Prophylactic vaccines. In addition to the therapeutic vaccines 

 for the treatment of the declared disease, vaccines are also employed 

 for prevention of disease. The preventive or prophylactic vaccines 

 may be : 



(1) Living, but attenuated, cultures, e.g. anthrax and cholera. 



1 See Harris, Practitioner, May 1908, p. 647. 



