350 A MANUAL OF BACTERIOLOGY 



the temperature at the time of inoculation is 2-5 F. above the 

 normal. In such cases, if there be any suspicious clinical signs to 

 assist, reliance may be placed upon the local swelling. 



(6) In animals the agglutination reaction is stated by Moore 

 and Taylor l to give accurate results. In man this test might 

 give an inconclusive result (see ante). 



(7) In the tissues the glanders bacillus is difficult to demonstrate. 

 Sections may be stained for half an hour with carbol methylene- 

 blue, treated with 4 per cent, acetic for a few seconds, washed, and 

 rapidly dehydrated with alcohol, cleared and mounted. McFadyean 

 recommends, after treating with acetic and washing, flooding with 

 a saturated solution of tannic acid in water for fifteen minutes, 

 washing, counter-staining in a 1 per cent, aqueous solution of acid 

 fuchsin for fifteen to thirty seconds, washing, dehydrating, and 

 clearing in cedar oil. 



Twort's method may also be employed (see section on Amoeba 

 coli, " Clinical Diagnosis "). 



1 Journ. of Infect. Diseases, Sup. No. 3, May 1907, p. 85. 



