464 A MANUAL OF BACTERIOLOGY 



observed. These were usually in pairs of unequal size, 

 the largest measuring about 16 /*, surrounded by a well- 

 defined capsule, and containing a granular protoplasm in 

 which a vacuole was present. Clinically, the case had 

 been regarded as one of scrofuloderma, but no tubercle 

 bacilli could be found. 



Numerous cases of blastomycetic dermatitis have now 

 been recognised, and several instances of general systemic 

 blastomycetic infection have been recorded. 



Granulomatous tumours occurring in epidemics among 

 horses in Japan, France, and Italy are also caused by 

 Blastomycetes. 



Clinical Examination (Pathogenic Yeasts, etc.) 



The cells can be well seen in the fresh state in the teased-up 

 tissues mounted in water or glycerin. 



Curtis recommends staining in carbol-thionine blue, and for 

 sections, picro- carmine. 



Busse's method for sections is as follows : 



1. Haematoxylin solution for fifteen minutes. 



2. Wash in distilled water. 



3. Counter-stain in weak carbol-fuchsin (1 : 20) for thirty minutes 

 to twenty -four hours. 



4. Decolorise in 95 per cent, alcohol for fifteen seconds to one 

 minute. 



5. Absolute alcohol, xylol, mount in Canada balsam. 

 Gilchrist recommends treating the sections with 10 per cent. 



caustic potash solution and examining in 50 per cent, glycerin 

 without staining. 



Brayton recommends that small pieces of the tissues should be 

 excised from the growing margin, treated with ether for two to 

 five minutes, macerated in 20 to 30 per cent, caustic potash solution 

 for five to ten minutes, and then examined without staining. 

 Cultures may be readily obtained, with a little care, preferably on 

 beer-wort gelatin or maltose agar. 



