540 



A MANUAL OF BACTERIOLOGY 



a month. This method does away with the necessity for 

 the daily inoculation of rabbits, a rabbit being inoculated 

 occasionally as required. The system of dosage employed 

 at the various anti-rabic stations differs somewhat ; the 

 following is that employed at Lille, 2 mm. of cord being 

 emulsified in 5 c.c. of sterile broth, or physiological salt 

 solution : 



ORDINARY TREATMENT. 



ORDINARY TREATMENT. 



At Buda-Pesth a dilution method has been employed ; 

 instead of drying the cords, an emulsion is made with 

 the fresh cord, and this emulsion is considerably diluted 

 for the earlier doses, dilutions of 1 in 10,000 to 1 in 6000, 

 corresponding to cords dried for from fourteen to eight 

 days. Semple * has found that a carbolised emulsion 

 of the cord may be employed as the inoculating agent. 

 An 8 per cent, emulsion of the cord in physiological salt 

 solution with 1 per cent, carbolic acid is kept at 37 C. 

 for twenty-four hours. At the end of this time an equal 

 volume of physiological salt solution is added and the 

 emulsion bottled aseptically. This vaccine will keep for 

 months. 



Undoubtedly the Pasteur inoculations will protect 

 animals from rabies, the duration of immunity after 



1 Sc. Mem. Gov. of India, No. 44. 



