552 A MANUAL OF BACTERIOLOGY 



The monkey and rabbit are both susceptible to vaccinia ; 

 in the latter animal the pustules are mature on the third 

 day and immunity is acquired by the sixth day. 



Ferroni and Massari state that appearances similar to 

 those described by Guarnieri can be obtained in cornese 

 inflamed by croton oil or Indian ink, and therefore believe 

 that the so-called parasites are derived from the nuclei 

 or from emigrated leucocytes. Salmon considers that the 

 so-called parasites in vaccinia and variola are more or less 

 condensed balls of chromatin of extra- epithelial origin 

 derived from the migratory polynuclear leucocytes. 

 According to von Prowazek these cell inclusions (the 

 Guarnieri bodies, etc.) in this and other conditions (e.q. 



'I \ ij 



scarlatina) are not parasites, but consist of plastin and 

 nuclease, and are derived from the cells in which they occur. 



De Korte 1 has observed in the variolous and vaccine 

 vesicles before maturation large amoeboid bodies (10 /u), 

 which he believes to be protozoa (Sporidium vaccinate). 

 In vaccine lymph refractile motile granules occur in 

 abundance, believed by De Korte to be spores. 



Fornet 2 by treating variola or vaccine lymph with ether 

 finds a stage when all the bacteria are killed but the specific 

 virus is uninjured. By inoculating this etherised lymph 

 into nutrient broth and keeping at 37 C., the broth culture 

 inoculated in man produces typical vesicles even after 

 two months incubation, and moreover the culture can 

 be carried on from tube to tube. In the broth, minute 

 rounded bodies can be detected which may be the specific 

 micro-organism. 



The relationship of vaccinia to variola has been a very 

 vexed question. With few exceptions (Ceely, Hime, 

 Simpson, Klein, King, Copeman) attempts to inoculate 



1 Trans. Path. Soc. Lond., vol. Ivi, 1905, p. 172. 



2 Trans. XVIIih Internal. Cong. Med. Lond., 1913, Sect, iv, pt. ii, 

 p. 119. 



