EXPERIMENTAL INOCULATION. 275 



Schutz, who, after one doubtful experiment, successfully 

 inoculated two horses in this way, the cultures used having 

 been grown for several generations outside the body. In 

 a few days swellings formed at the sites of inoculation, 

 and later broke down into unhealthy -looking ulcers. 

 There was the usual involvement of the lymphatic vessels 

 and glands, and symptoms due to affection of the nasal 

 mucous membrane also appeared after some time, there 

 being the characteristic discharge. One of the animals 

 died; after a few weeks the other, showing symptoms of 

 cachexia, was killed. In both animals, in addition to 

 ulcerations on the surface with involvement of the 

 lymphatics, there were found post mortem, nodules in the 

 lungs, softened deposits in the muscles, and also affection 

 of the nasal mucous membrane, nodules, and irregular 

 ulcerations. The ass is even more susceptible than the 

 horse, the disease in the former running a more rapid 

 course, but with similar lesions. The ass can be readily 

 infected by simple scarification and inoculation with glanders 

 secretion, etc. (Nocard). 



Of small animals, field-mice and guinea-pigs are the 

 most susceptible. Strangely enough, house-mice and white 

 mice enjoy an almost complete immunity. In field-mice 

 subcutaneous inoculation is followed by a very rapid 

 disease, usually leading to death within eight days, the 

 organisms becoming generalised and producing numerous 

 minute nodules, especially in the spleen, lungs, and liver. 

 In the guinea-pig the disease is less acute, though secondary 

 nodules in internal organs are usually present in consider- 

 able numbers. At the site of inoculation an inflammatory 

 swelling forms, which soon softens and breaks down, leading 

 to the formation of an irregular crateriform ulcer with 

 indurated margins. The lymphatic vessels become in- 

 filtrated, and the corresponding lymphatic glands become 

 enlarged to the size of peas or small nuts, softened, and 

 semi-purulent. The animal sometimes dies in two or three 

 weeks, sometimes not for several weeks. Secondary 

 nodules, in varying numbers in different cases, may be 



