3 8 TETANUS. 



" 



what is usually described as the drumstick form (Figs. 95, 



98). In a speci- 

 men stained with 

 a watery solution 

 of gentian-violet or 

 methylene-blue, the 

 spores are un- 

 coloured except at 

 the periphery, so 

 jaJ that the appearance 

 of a small ring is 

 produced ; if a 

 powerful stain such 

 as carbol-fuchsin be 

 applied for some 

 time, the spores 



FIG. 97. Spiral composed of numerous become deeolv 

 twisted flagella of the tetanus bacillus. ^i 1 ri 



Stained by Rd. Muir's method. x 1000. col OUred like the 



bacilli. Further, 

 they may become 

 free in the culture 

 medium. They can 

 be stained by the 



* 



appropriate methods. r 



Isolation. The ^jf 



isolation of the te- ^ 



tanus bacillus is some- x^. I 



what difficult. By ^-/ **" V 



inoculation experi- \ """^v ' 



ments in animals, its / V 



natural habitat has _^ t 



been proved to be /** ^ 



garden soil, and t 

 especially the con- 

 tents of dung heaps, 



where it probably FlG - 98. Tetanus bacilli; some of which 



leads a saprophytic possess sp res ' From a culture in s lucose 



agar, incubated for three days at 37 C 



existence, though Its Stained with carbol-fuchsin x 1000 



