VIBRIO METCHNIKOVL 



427 



Koch's spirillum (Fig. 109). It is actively motile, and has 

 the same staining reactions. Its growth in peptone-gelatine 

 also closely resembles that of the cholera organism, though 

 it produces liquefaction more rapidly (Fig. no, A). In 

 gelatine plates the young 

 colonies are, however, 

 smoother and more 

 circular. After liquefac- 

 tion occurs, some of the 

 colonies are almost 

 identical in appearance 

 with those of the cholera 

 organism, whilst others 

 show more uniformly 

 turbid contents. In 

 puncture cultures the 

 growth takes place more 

 rapidly, but in appear- 

 ance closely resembles 

 that of the cholera 

 organism a few days 

 older. Its growth in 

 peptone solution too is 

 closely similar, and it 

 also gives the cholera-red 

 reaction. 



This organism can, 

 however, be readily FlG I 7 . -Puncture cultures in pep- 

 distinguished from the tone-gelatine. 



Cholera Organism by the A - Metchnikoff's spirillum. Five days' 

 effects Of inoculation On B. Finkler and Prior's spirillum. Four 



animals, especially on days>s 



pigeons and guinea-pigs. Subcutaneous inoculation of small 

 quantities of pure culture in pigeons is followed by acute 

 inflammatory swelling with degeneration of the subjacent 

 muscles ; and septicaemia occurs, which produces a fatal 

 result usually within twenty-four hours. Inoculation with the 

 same quantity of cholera organism produces practically no 



