BACILLUS ICTEROIDES. 455 



becomes in twelve hours surrounded by a halo of white, 

 opaque, pearly growth of higher level than the central part. 

 In a few days the growth at the lower temperature becomes 

 liquid in character and runs slowly down the medium as a drop 

 of melted paraffin would do (Fig. 118). Growth also takes 

 place in bouillon and blood serum. On potatoes there is 

 a fine transparent pellicle which does not alter its colour 

 with age. Milk is curdled, but only after some weeks. 

 The bacillus ferments glucose but not lactose. It gives 

 a feeble indol reaction. 



Sanarelli investigated twelve cases of yellow fever 

 and found the B. icteroides present in relatively small 

 numbers in six. It appeared chiefly in the capillaries 

 of the liver and kidneys, rarely in other parts of the 

 body. The methods applied in the case of the gastro- 

 intestinal tract are not given, but it was never found 

 there. 



Mice, guinea-pigs and rabbits succumbed to subcu- 

 taneous injection of the bacilli, but the results were not 

 characteristic. In the dog sometimes death occurred after 

 intravenous injection in a few hours, sometimes in from 

 eight to twenty-one days. The symptoms were sickness, 

 continuous loss of weight, anuria, diarrhoea, it might be 

 sanguineous, and jaundice. Post mortem there were 

 fatty degeneration of the liver and a grave glomerulo- 

 nephritis, the intestinal walls were hyperaemic, and the 

 lumen filled with a coffee -coloured matter. The blood 

 contained an excess of urea and the B. icteroides was 

 more or less widely present in the body. 



Bouillon cultures twenty days old when filtered germ- 

 free and injected subcutaneously do not give rise in the 

 dog to a characteristic illness. Cultures killed by ether 

 and injected intravenously cause practically the same 

 effects as living cultures. Sanarelli states that sterile 

 bouillon cultures when injected in man subcutaneously or 

 intravenously give rise to all the symptoms of yellow 

 fever. 



If the latter statement be correct there can be little 



