526 MALARIAL FEVER. 



itself out between two cover-glasses, which are separated 

 by sliding the one on the other. The films are then 

 allowed to dry. A very good method is that of Manson, 

 who catches the drop of blood on a piece of gutta-percha 

 tissue, and then makes a film on a clean slide by drawing 

 the blood over the surface. The dried films are then fixed 

 by one of the methods already given (p. 96), or by placing 

 in absolute alcohol for five minutes (Manson). They may 

 be stained by a saturated watery solution of methylene-blue 

 for five minutes, or by Ehrlich-Biondi fluid for half an 

 hour. A double stain may be obtained by staining first 

 with a i per cent watery solution of eosin for five minutes, 

 then washing well in water, and thereafter staining for about 

 a minute with a saturated watery solution of methylene-blue ; 

 the red corpuscles are red, the parasites and nuclei of 

 leucocytes are coloured with the blue. After being stained 

 the films are washed in water, dried, and mounted in 

 balsam ; those stained with Ehrlich-Biondi fluid are for 

 some purposes best examined in the dry condition, the 

 cover-glass being fixed at its margins to the slide by 

 balsam, the film downwards, but not in contact with the 

 slide. 



