METHODS OF EXAMINATION. 533 



A drop is placed on a slide without the addition of any 

 reagent, a cover-glass is placed over it and the preparation 

 is examined in the ordinary way or on a hot stage, prefer- 

 ably by the latter method, as the movements of the amoebae 

 are more active and it is difficult to recognise them when 

 they are at rest. Dried films are not suitable, as in the 

 preparation of these the amoebae become broken down ; 

 but films may be fixed with corrosive sublimate or other 

 fixative (vide p. 97). In sections of tissue the amoebae may- 

 be stained by methylene-blue, by safranin, by haematoxylin, 

 and eosin, etc. 



