ANTIBACTERICIDAL PROPERTIES OP SNAKE VENOM 217 



From this it may be concluded that the specimen of venom employed by 

 Flexner and Noguchi destroyed the bactericidal properties of dog serum 

 against B. typhosus when added in the proportion of 0.00005 g m - f venom 

 to i c.c. of serum, and that 0.00002 gm. in the same quantity of serum 

 was practically without action. 



Flexner and Noguchi found that o.oooi to 0.0006 gm. of copperhead 

 venom added to i c.c. of Necturus serum removed only a part of its bacteri- 

 cidal properties against B. coll and B. typhosus, but its inaction was very 

 remarkable in contrast to the other serums they employed. It may be 

 remarked that Necturus is highly refractory to venom. An animal weighing 

 250 gm. received without ill effect 0.05 gm. venom, equivalent to 160 mini- 

 mal lethal doses for the guinea-pig of equal weight. 



The effect of heat upon venom in relation to its action upon the bactericidal 

 properties is of interest. For this purpose cobra, water-moccasin, copperhead, 

 and rattlesnake venoms were studied. Temperatures varying from 75 to 

 90 C. were employed, and the heated venoms were mixed with the stream- 

 ing blood in Nuttall bulbs and with the separated serum. The venom was 

 kept at the lower temperature (75 C.) for 30 minutes and at the higher 

 temperature (90 C.) for 15 minutes. The heated venom acts just as the 

 unheated, except in the case of crotalus venom, the effect of which is some- 

 what diminished at the higher temperature (90 C.). 



Finally, Flexner and Noguchi attributed the antibactericidal properties of 

 various venoms to the fixation or inactivation of bacteriolytic complements, 

 but not to any perceptible alterations on the part of intermediary bodies 

 (amboceptors) of these serums. 



In 1905 Noc * made interesting observations concerning the anticomple- 

 mentary property of cobra venom. He found that the bacteriolytic actions 

 of the fresh serums of the rabbit, horse, guinea-pig, rat, and man are com- 

 pletely annihilated by this venom, which is due to the fixation of comple- 

 ments by venom. He employed haemolytic reaction to demonstrate the 

 absence of complements from the venomized serum. Normal rat serum is 

 haemolytic upon the corpuscles of horse blood, and this was used as the test 

 reaction. He prepared 6 different combinations: 



TABLE 19. 



Normal rat serum 0.5 c.c. 



Normal rat serum 0.5 c.c. +0.0005 gm. cobra venom, after 15 min. i c.c. antivenin. 



Normal rat serum 0.5 c.c. + o.ooi gm. cobra venom, after 15 min. a c.c. antivenin. 



Cobra venom o.ooi gm. 

 (5! Antivenin i c.c. 

 (6) Normal rat serum 0.5 c.c. + i c.c. antivenin. 



Then 2 drops of defibrinated horse blood were introduced into each tube 

 and incubated at 37 C. 



The results: Complete haemolysis in a few minutes in (i) and (6). In (4) 

 haemolysis completed in i hour, but no haemolysis in (2) and (3), showing 

 that 0.5 and o.ooi gm. of cobra venom completely destroyed alexine in 15 



1 Noc. Ann. Inst. Pasteur, 1905, XIX, 309. 



