CHAPTER XXV. 

 INTERACTIONS BETWEEN VENOM AND ANTIVENIN. 



ESTABLISHMENT OF THE CHEMICAL NATURE OF THE VENOM-ANTIVENIN 



REACTION. 



The poisonous constituents of snake venom have one of the most important 

 peculiarities of the class of substances known as toxins, namely, they stimu- 

 late in the body of susceptible animals the formation of specific anti-bodies 

 when introduced into the latter in sublethal quantities. This was proven by 

 Sewall even before the discovery of Behring and Kitasato of the antitoxin 

 for diphtheria, and fully confirmed and extended by Phisalix, Bertrand, 

 Calmette, Fraser, and many other later investigators. Thus the fact that 

 animals immunized with snake venom acquire a higher resistance to the 

 effects of the same venom, and that this acquired immunity can easily be 

 transmitted to a normal animal through the introduction of the blood serum 

 of the immunized animal, has been proven beyond doubt. But what is the 

 cause of such protection? Is it due to the formation of new substances in 

 the body of the immunized animal, capable of neutralizing the toxic substances 

 of the venom, or is it due to a mere physiological tolerance of the vulnerable 

 cells to the noxious effects of the poison? 



Sewall's experiments fail to decide this point, but the work of Calmette 

 and Fraser has already shown that this is not a mere toleration on the part of 

 cells, but is due to the acquisition of a new property by the blood serum 

 perhaps including also certain other body fluids as the result of artificial 

 immunization, namely, the antivenomous property. In other words, the 

 antitoxin has developed in the immunized animal. Thus the protection 

 afforded by active as well as passive immunities is the work of antitoxin. To 

 this every investigator in this field agrees. 



The next question, which has a broader bearing upon the mechanism 

 of antitoxin immunity in general, is whether the counteracting property of 

 antitoxin against toxin is effected directly or indirectly through the coopera- 

 tion of cellular elements of the animal to be protected. 



By the first hypothesis venom is directly acted upon by the antivenin with- 

 out the aid of vital process of the cells, like an acid upon a base. By the 

 second hypothesis the antitoxin renders the susceptible cells less sensitive 

 to the toxin without attacking the toxin directly. The former is known in 

 immunity as the chemical theory, while the latter view is the cellular or vital 

 theory. 



A clear-cut decision on this point has for a long time been almost impossible, 

 as there were no means of separating toxin and antitoxin from the mixture of 

 both, once made outside the body. All toxins and antitoxins lose their activi- 



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