92 MORPHINE. 



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MORPHINE. 



1. Into the anterior lymph sac of a male frog inject I mil of a 4% solution Mor- 

 phine Sulphate. Observe reactions for 2 hours in comparison with a normal frog of 

 equal weight and with another frog of same weight which has had a like dose 2 hours 

 previously. Note how these animals respond to sundry stimuli akin to that experienced 

 in their normal environment. 



2. Take 2 frogs of equal weight and condition. Compare their reactions when 

 one has I mil of a 4% solution Morphine Sulphate injected into its stomach, while the 

 other has a like dose injected into its anterior lymph sac. 



3. Pith each brain of 2 frogs of equal weight and condition. By leg lymph sac 

 administer to one i mil of a 4% solution Morphine Sulphate, to the other a like dose, 

 but containing in addition 0.002 Gm. Atropine Sulphate. Arrange for heart tracings, 

 and secure intermittent comparative records for the next two hours. Frogs must be 

 kept moist, and at a constant cool temperature. What do your graphs seem to indicate? 



4. Take a cat, a rabbit, and a small dog. Obtain an average of 3 observations on 

 each of heart-rate and respiration. To the rabbit give hypodermically of Morphine 

 Sulphate 0.020 Gm. per kilogram body-weight ; to the cat the same way, 0.050 Gm. per 

 kilo ; to the dog the same way, 0.020 Gm. per kilo. Carefully observe the comparative 

 sequence of phenomena elicited by Morphine Sulphate on these types of mammals. 



5. When the animals in Experiment No. 4 show evidences of narcotism, lightly 

 etherize them, place on animal-board, connect with recording apparatus. Remove the 

 ether. Continue records until death ensues, administering more of the Morphine if 

 necessary. (Sollmann gives the minimal fatal dose (M. F. D.) of Morphine as fol- 

 lows : dog, 0.4 Gm. x kg. ; cat, 0.04 to 0.08 Gm. x kg. ; rabbit, 0.3 Gm. x kg. ) 



6. Select 3 fasting rabbits of like weight and condition. Administer by stomach, 

 to one, Morphine Sulphate 0.020 Gm. x kg. ; to the second, Tincture Opium, 2 mils x 

 kg. ; to the third, Morphine Sulphate, 0.020 Gm. and Atropine Sulphate 0.002 Gm. x 

 kg. Compare reactions throughout laboratory period. 



7. Let a member of the class volunteer to take 0.020 Gm. Morphine Sulphate. 

 Let him be seated in a comfortable position, in a steamer chair for example. Secure 

 his normal pulse, respiration, blood-pressure and temperature. With sesthesiometer 

 test his forearm and forehead for tactile discrimination. Test his reaction time for 

 sight, hearing, feeling. Make speed tests of his mental activity, using the antonym 

 test, the test for imagery, and the multiplication test. Let him take the drug in solu- 

 tion. Take systemic observations every 10 minutes, and mental and sesthesic tests 

 every half hour. 



8. In the hospital, study various cases to whom Morphine has been administered 

 by the attending physician. 



9. In the psychopathic ward, study cases under treatment for the relief or cure 

 of morphinism. 



10. Consult recent literature for at least four discussions of the pharmacology of 

 Morphine. Write up a brief resume of your laboratory findings, checking the same 

 with citations from your readings, either confirmative or otherwise. 



