104 ATROPINE. 



ATROPINE. 



1. To a frog administer by anterior lymph sac o.oio Gm. Atropine Sulphate. 

 Observe respiration and reflexes in comparison with a frog of equal weight and condi- 

 tion similarly drugged one hour previously. 



2. Expose the heart of a pithed frog. Secure a normal cardiac tracing, one as 

 affected by weak stimulation of the vagus, and another as affected by stimulation of 

 the sinus venosus. Apply Atropine Sulphate solution, 0.1%, continuing the record. 

 After a few minutes, apply the weak current as before, noting any variation from the 

 preceding graph. How may these variations be explained? 



3. Take two cats of equal weight and condition. Obtain their heart-rates and 

 respirations, and note the size and condition of their pupils. To each administer 0.04 

 mg. x kg. Atropine Sulphate, giving it by stomach to one, and hypodermically to the 

 other. Make careful comparative observations for 2 to 3 hours. 



4. Etherize a cat, arrange connections for registering circulatory and respiratory 

 phenomena. Secure ether norms, and records resulting from stimulating vagus with 

 minimal induced electric currents. Into jugular vein slowly inject 0.05 mg. x kg. 

 Atropine Sulphate, making continuous tracings in the meanwhile. When a pronounced 

 effect is apparent, again stimulate the vagi with the same strength current as before. 

 What results? 



Continue injection of Atropine Solution until death ensues, noting all important 

 phenomena. 



5. On two students of similar weight and condition secure normals of pulse-rate, 

 respiration, blood-pressure, temperature, and pupil. To one administer 0.8 mg. Atro- 

 pine Sulphate in solution by mouth; to the other, i mil Tincture Belladonna (=0.3 

 mg. total alkaloids). Make comparative observations of all systems for the next two 

 hours. 



6. Take 2 rabbits of equal weight and condition. Hypodermically administer to 

 one 2 mg. x kg. Atropine Sulphate, to the other give 1.5 mg. x kg. Hyoscine (Scopo- 

 lamine) Hydrobromide. Make critical comparison for the next 2 hours. 



7. Try out a similar experiment on 2 students of approximate weight and condi- 

 tion, using 0.5 mg. (per total weight) Atropine Sulphate, and 0.8 mg. Hyoscine 

 Hydrobromide . 



8. In the hospital assignment endeavor to ascertain any reactions, in various ill- 

 nesses, reasonably due to the administration of Atropine. 



