EPINEPHRIN. 135 



EPINEPHRIN. 



1. Expose the heart of a pithed frog; connect with heart lever, and obtain a nor- 

 mal tracing. Apply to the heart 2 minims Epinephrin solution, i to 1000, and con- 

 tinue tracings, noting whatever effect may be produced. 



2. Repeat Experiment No. i, except to inject o.i mil of the Epinephrin solution 

 into the thigh lymph sac. 



3. Obtain a normal heart tracing from a pithed frog. Administer by lymph sac 

 0.2 mil o.oi sol, Atropine Sulphate. Obtain brief records every few minutes. After 

 l /2 hour, apply some Epinephrin solution, and observe resulting tracing. Compare it 

 with that obtained in Experiment No. i. 



4. Etherize a dog, and make connections with respiratory and circulatory record- 

 ing apparatus. To one carotid attach a mercury manometer ; to the other attach a 

 Hiirthle manometer, and adjust it for marked upward excursion. Secure normals, 

 then, with continuous drum, with hypodermic needle inject into jugular vein 0.05 mil 

 per kg. Epinephrin solution, i to 1000, marking drum at moment of injection. 



When normal conditions return, repeat injection, comparing time and extent of 

 response. 



When normal conditions return, readjust the Hiirthle manometer for downward 

 excursions. Administer via jugular vein with hypodermic 0.5 mil per kg. of Nitro- 

 glycerin, i to 1000 solution. When blood-pressure is at its lowest point, inject 0.05 

 mil per kg. Epinephrin solution, I to 1000. 



When normal conditions return, reverse the preceding experiment. 



Cause death with Epinephrin solution, noting phenomena. 



5. Select a group of three students of equivalent weight, temperament, and con- 

 dition. Record their normals; then administer 0.5 mil Epinephrin solution, i to 1000 



to one by mouth, to a second subcutaneously, to a third intramuscularly. Take note 

 of all reactions. Compare with others. See particularly if reaction seems to be modi- 

 fied in any way by temperament or physique. 



6. Study the uses of Epinephrin in the operative clinics of ophthalmology and 

 rhinology. 



Ascertain also in what ward, and for what conditions, the further utility of 

 Epinephrin is being studied. Learn the present status of such investigations, and 

 compare results with what you have thus far obtained. 



7. Marshal in systematic form the leading facts known about the pharmacody- 

 namics of Epinephrin, giving your authority in each case. 



EXPERIMENTS No. i, 2 AND- 3. 

 Kymograph records. Frog: 



