140 THE NITRITE SERIES. 



THE NITRITE SERIES. 



1. Prepare a frog for heart tracings, and secure normals on both the slow and 

 fast drum. Into the anterior lymph sac inject o.i mg. Nitroglycerin. Take frequent 

 sample graphs on both drums. 



2. Place a cat in the etherizing box, and into the cone drop 3 minims Amyl Nitrite. 

 Administer immediately for y 2 minute, then quickly place cat under a cage, and watch 

 her recovery. 



3. Obtain the normals of pulse and respiration of a rabbit ; then administer sub- 

 cutaneously 0.5 mg. Nitroglycerin. Plot your findings on the chart. 



4. Etherize a cat; secure the usual registrations. Keeping apparatus running 

 (Hiirthle manometer should be adjusted for low drop), drop into ether cone 3 minims 

 Amyl Nitrite, and obtain the registered effect. This may be repeated several times. 



5. When the normal returns (in Experiment No. 4), administer to the cat 0.25 

 mg. x kg. Nitroglycerin intraperitoneally. Keep constant drum until registration 

 marks normal. 



6. With the return of normal, administer the same dose intravenously. When 

 the drug action is at its maximum, administer by jugular vein 0.3 mil Epinephrin solu- 

 tion, i to 1000. 



7. Form a group of six. A, the subject, is to inhale 3 minims Amyl Nitrite, gaz- 

 ing meanwhile at a black spot on a large white surface ; B, to take continuous pulse- 

 count, announcing the count in continual 5-second intervals ; C, to record the pulse as 

 announced ; D, to take continuous blood-pressure readings, quickly following the rapid 

 variations ; E, to record the pressure findings ; F, to obtain sphygmograms and to note 

 subject's facial appearance. Let subject break an Amyl Nitrite pearl in a towel, and 

 immediately inhale the fumes, continuing for */> minute unless he feels too dizzy. Both 

 reaction and recovery will be rapid, so observers must maintain keen attention. 



8. Nitroglycerin in therapeutic doses is not dangerous, but it sometimes produces 

 a rather severe headache; this lessens with tolerance. Therefore if one accustomed 

 somewhat to the drug, or if some one else will volunteer, let the usual observations 

 be made concerning the effects of Nitroglycerin. Dose, 0.5 mg. 



9. In the hospital assignment, determine the reactions of Nitroglycerin in the 

 several instances where it may have been prescribed. Compare these reactions with 

 those you have observed in the laboratory. Endeavor to ascertain, also, how extent 

 and duration of Nitroglycerin action may be modified in any given case by habituation. 



10. Consulting the literature, ascertain the rationale of the use of Nitroglycerin, 

 and seek to explain any apparent discrepancies. 



EXPERIMENT No. i. 

 Graphs. Frog : 



